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Organization of neurochemical interactions in young and older brains as revealed with a network approach: evidence from proton magnetic resonance spectroscopy (1H-MRS)

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After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 µg ml –1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added