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(a-v-a-viii) After 90 min oxygen-glucose deprivation, cells were incubated with normal medium containing (25 mM glucose) and 21 % O2 for different reperfusion time, 30 min (a-v), 60 min (a-vi), 90 min (a-vii), 120 min (a-viii), subsequent, LJ-GSH (10 μM) were added to cells for another 30 min incubation