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SH-SY5Y cells were lysed in ice-cold RIPA buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 5 mM EDTA, 1% IGEPAL-CA-630, 3 mM Na 3 VO 4 , 3 mM PMSF), supplemented with 1% protease inhibitor cocktail and 1% phosphatase inhibitor cocktail

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Clinical research has suggested that the levels of FOXO proteins in the central nervous system may be altered in research models of neurodegenerative disorders (NDDs)