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doi: 10.1016/j.toxlet.2003.12.074

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The rate of migration was measured by quantifying the % of wound closure area, determined using the software ImageJ, according to the formula: % of wound closure = [(At = 0 h - At = Δ h)/At = 0 h] × 100% Statistical analysis Data is represented as the mean ± standard error of the mean (SEM), taken over ≥ 3 independent experiments, with ≥ 3 technical replicates per experiment, unless otherwise stated