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All experimental groups were processed simultaneously to ensure staining consistency

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While at higher concentrations nitric oxide (NO) is detrimental and contributes to neuroinflammation and neurodegeneration, at normal physiological levels, the role of NO is integral within the brain for neurotransmission (modulates serotonin, dopamine, and norepinephrine), neuromodulation, and synaptic plasticity ( The effects of PYC on stimulated macrophage cell cultures to mimic the immune response identified that the most active metabolite of PYC, M1 (δ-(3,4-dihydroxyphenyl)-γ-valerolactone), mediated anti-inflammatory effects by impeding nitric oxide generation and inducible nitric oxide synthase (iNOS) upregulation production ( via the GLUT-1-type glucose transporter ( in vitro study utilizing cultured B16 melanoma cells found that while PYC suppressed reactive species in their assays, glutathione was enhanced (Wu and Batist, 2013)