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doi: 10.1038/mi.2014.23 118 ChenLYuYLiuEDuanLZhuDChenJet al
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The following xiSEARCH configuration was used for the search for the non-oxidized samples: carbamidomethyl (C, 57.021464, variable), deamidation (N, 115.026397 (delta mass), variable), oxidation (M, 147.035395 (delta mass), variable), pyroglutamate (N-terminal Q, -17.026549105, variable), 4-ONE-Schiff base formation (K, 136.05242, variable), dihydroxyl (W/M, 31.989829, variable), kynurenine formation (W, 3.994915, variable), 4-ONE-Unreacted (K, 154.09938, linear), water loss (S/T/D/E/C-terminal, 18.01056027), ammonia loss (R/K/N/Q/N-terminal, 17.02654493), methionine hydroxylation loss (M, 63.99828547), trypsin digest, 3 missed cleavages allowed, 4-ONE crosslink (K/N-terminal to C, 118.07825), non-covalent crosslink, MS1 tolerance of 2.0 ppm, MS2 tolerance of 5.0 ppm, and 3 max modifications per peptide